Anti-DDX4 antibody (550-600 aa) (STJ13101035)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 550-600 of human DDX4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
General Information
| Short Description | Rabbit polyclonal anti-DDX4 (550-600 aa) for use in IHC and WB in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | DDX4 |
| Gene ID | 54514 |
| Uniprot ID | DDX4_HUMAN |
| Immunogen | A synthetic peptide from aa region 550-600 of human DDX4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Immunogen Region | 550-600 aa |
| Specificity | Specific for DDX4. |
Additional Info
| Function | ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs. |
| Protein Name | Probable Atp-Dependent Rna Helicase Ddx4Dead Box Protein 4Vasa Homolog |
| Database Links | Reactome: R-HSA-5601884Reactome: R-HSA-9696264 |
| Cellular Localisation | CytoplasmPerinuclear RegionComponent Of The Meiotic NuageAlso Named P GranuleA Germ-Cell-Specific Organelle Required To Repress Transposon Activity During Meiosis |
| Alternative Antibody Names | Anti-Probable Atp-Dependent Rna Helicase Ddx4 antibodyAnti-Dead Box Protein 4 antibodyAnti-Vasa Homolog antibodyAnti-DDX4 antibodyAnti-VASA antibody |
Information sourced from Uniprot.org