| Host: |
Mouse |
| Applications: |
WB/IHC/IF/FC/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Probable ATP-dependent RNA helicase DDX4 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
2F9H5 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC 1:200-1:1000IF 1:200-1:1000FC 1:200-1:400ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DDX4 |
| Gene ID: |
54514 |
| Uniprot ID: |
DDX4_HUMAN |
| Specificity: |
DDX4 Monoclonal Antibody detects endogenous levels of DDX4 protein. |
| Immunogen: |
Purified recombinant fragment of human DDX4 expressed in E. Coli |
| Function | ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs. |
| Protein Name | Probable Atp-Dependent Rna Helicase Ddx4Dead Box Protein 4Vasa Homolog |
| Database Links | Reactome: R-HSA-5601884Reactome: R-HSA-9696264 |
| Cellular Localisation | CytoplasmPerinuclear RegionComponent Of The Meiotic NuageAlso Named P GranuleA Germ-Cell-Specific Organelle Required To Repress Transposon Activity During Meiosis |
| Alternative Antibody Names | Anti-Probable Atp-Dependent Rna Helicase Ddx4 antibodyAnti-Dead Box Protein 4 antibodyAnti-Vasa Homolog antibodyAnti-DDX4 antibodyAnti-VASA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
