• Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-DDX21 antibody (287-337 aa) (STJ195748)

SKU:
STJ195748

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nucleolar RNA helicase 2 (287-337 aa) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX21
Gene ID: 9188
Uniprot ID: DDX21_HUMAN
Immunogen Region: 287-337 aa
Specificity: This antibody detects endogenous levels of DDX21 at Human/Mouse/Rat
Immunogen: Synthesized peptide derived from the human DDX21 at the amino acid range 287-337
Function RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs. In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes. In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes. Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77'. Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Together with SIRT7, required to prevent R-loop-associated DNA damage and transcription-associated genomic instability: deacetylation by SIRT7 activates the helicase activity, thereby overcoming R-loop-mediated stalling of RNA polymerases. Involved in rRNA processing. May bind to specific miRNA hairpins. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1.
Protein Name Nucleolar Rna Helicase 2
Dead Box Protein 21
Gu-Alpha
Nucleolar Rna Helicase Gu
Nucleolar Rna Helicase Ii
Rh Ii/Gu
Database Links Reactome: R-HSA-5250924
Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Cytoplasm
Cytosol
Mitochondrion
Present Both In Nucleolus And Nucleoplasm
Interaction With Jun Promotes Translocation From The Nucleolus To The Nucleoplasm
Interaction With Wdr46 Is Required For Localization To The Nucleolus
Colocalizes In The Cytosol With Ddx1
Dhx36 And Ticam1
The Multi-Helicase-Ticam1 Complex May Translocate To The Mitochondria Upon Poly(I:c) Rna Ligand Stimulation
Alternative Antibody Names Anti-Nucleolar Rna Helicase 2 antibody
Anti-Dead Box Protein 21 antibody
Anti-Gu-Alpha antibody
Anti-Nucleolar Rna Helicase Gu antibody
Anti-Nucleolar Rna Helicase Ii antibody
Anti-Rh Ii/Gu antibody
Anti-DDX21 antibody

Information sourced from Uniprot.org

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