| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DDX21 (1-180) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DDX21 |
| Gene ID: |
9188 |
| Uniprot ID: |
DDX21_HUMAN |
| Immunogen Region: |
1-180 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human DDX21 (NP_004719.2). |
| Immunogen Sequence: |
MPGKLRSDAGLESDTAMKKG ETLRKQTEEKEKKEKPKSDK TEEIAEEEETVFPKAKQVKK KAEPSEVDMNSPKSKKAKKK EEPSQNDISPKTKSLRKKKE PIEKKVVSSKTKKVTKNEEP SEEEIDAPKPKKMKKEKEMN GETREKSPKLKNGFPHPEPD CNPSEAASEESNSEIEQEIP |
| Post Translational Modifications | Acetylation by CREBBP/CBP inhibits the helicase activity. Deacetylation by SIRT7 promotes the helicase activity and overcomes R-loop-mediated stalling of RNA polymerases. |
| Function | RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs. In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes. In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes. Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77'. Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Together with SIRT7, required to prevent R-loop-associated DNA damage and transcription-associated genomic instability: deacetylation by SIRT7 activates the helicase activity, thereby overcoming R-loop-mediated stalling of RNA polymerases. Involved in rRNA processing. May bind to specific miRNA hairpins. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1. |
| Protein Name | Nucleolar Rna Helicase 2Dead Box Protein 21Gu-AlphaNucleolar Rna Helicase GuNucleolar Rna Helicase IiRh Ii/Gu |
| Database Links | Reactome: R-HSA-5250924Reactome: R-HSA-6791226 |
| Cellular Localisation | NucleusNucleolusNucleoplasmCytoplasmCytosolMitochondrionPresent Both In Nucleolus And NucleoplasmInteraction With Jun Promotes Translocation From The Nucleolus To The NucleoplasmInteraction With Wdr46 Is Required For Localization To The NucleolusColocalizes In The Cytosol With Ddx1Dhx36 And Ticam1The Multi-Helicase-Ticam1 Complex May Translocate To The Mitochondria Upon Poly(I:c) Rna Ligand Stimulation |
| Alternative Antibody Names | Anti-Nucleolar Rna Helicase 2 antibodyAnti-Dead Box Protein 21 antibodyAnti-Gu-Alpha antibodyAnti-Nucleolar Rna Helicase Gu antibodyAnti-Nucleolar Rna Helicase Ii antibodyAnti-Rh Ii/Gu antibodyAnti-DDX21 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
