Anti-DDX1 antibody [SRM13487] (STJ11108217)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal Atp-Dependent Rna Helicase Ddx1 antibody for use in WB, IHC-P, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM13487 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB, 1:10000-1:60000IHC-P, 1:1000-1:5000IP, 0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cellsELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay req |
| Formulation | PBS containing 50% Glycerol and 0.05% BSA, preserved with proclin300 or Sodium Azide (as specified on the Certificate of Analysis) , pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DDX1 |
| Gene ID | 1653 |
| Uniprot ID | DDX1_HUMAN |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Immunogen Sequence | Human |
Additional Info
| Post Translational Modifications | Phosphorylated by ATM kinase.phosphorylation is increased in response to ionizing radiation (IR). |
| Function | Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA. (Microbial infection) Required for HIV-1 Rev function as well as for HIV-1 and coronavirus IBV replication. Binds to the RRE sequence of HIV-1 mRNAs. (Microbial infection) Required for Coronavirus IBV replication. |
| Protein Name | Atp-Dependent Rna Helicase Ddx1Dead Box Protein 1Dead Box Protein RetinoblastomaDbp-Rb |
| Database Links | Reactome: R-HSA-6784531 |
| Cellular Localisation | NucleusCytoplasmCytoplasmic GranuleCytosolMitochondrionLocalized With Mbnl1Tial1 And Ybx1 In Stress Granules Upon StressLocalized With Cstf2 In Cleavage BodiesForms Large Aggregates Called Ddx1 BodiesRelocalized Into Multiple Foci (Ir-Induced Foci Or Irif) After Ir TreatmentA Process That Depends On The Presence Of Chromosomal Dna And/Or Rna-Dna DuplexesRelocalized At Sites Of Dna Double-Strand Breaks (Dsbs) In An Atm-Dependent Manner After Ir TreatmentColocalized With Rela In The Nucleus Upon Tnf-Alpha InductionEnters Into The Nucleus In Case Of Active Transcription While It Accumulates In Cytosol When Transcription Level Is LowColocalizes In The Cytosol With Ddx21Dhx36 And Ticam1Colocalizes In The Mitochondria With Ticam1 And Poly(I:c) Rna LigandThe Multi-Helicase-Ticam1 Complex May Translocate To The Mitochondria Upon Poly(I:c) Stimulation(Microbial Infection) Relocalized To The Cytoplasm With A Perinuclear Staining Pattern In Avian Infectious Bronchitis Virus (Ibv)-Infected CellsRequired For Proper Localization Of Hiv-1 Rev |
| Alternative Antibody Names | Anti-Atp-Dependent Rna Helicase Ddx1 antibodyAnti-Dead Box Protein 1 antibodyAnti-Dead Box Protein Retinoblastoma antibodyAnti-Dbp-Rb antibodyAnti-DDX1 antibody |
Information sourced from Uniprot.org