| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DDRGK domain-containing protein 1 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DDRGK1 |
| Gene ID: |
65992 |
| Uniprot ID: |
DDRGK_HUMAN |
| Specificity: |
This antibody detects endogenous levels of DDRGK1 at Human, Mouse |
| Immunogen: |
Synthesized peptide derived from human DDRGK1 |
| Post Translational Modifications | Ubiquitinated. Ubiquitination probably triggers proteasomal degradation and is negatively regulated by UFL1, the enzyme involved in the ufmylation of DDRGK1. Ufmylated.conjugated to ubiquitin-like protein UFM1, probably at Lys-267 by UFL1. The relevance of ufmylation is however unclear: as DDRGK1 acts as substrate adapters for ufmylation, it is uncertain whether ufmylation is a collatoral effect of ufmylation process or is required to regulate its activity. |
| Function | Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, which plays a key role in reticulophagy (also called ER-phagy). In response to endoplasmic reticulum stress, promotes recruitment of the E3 UFM1-protein ligase UFL1 to the endoplasmic reticulum membrane: in turn, UFL1 mediates ufmylation of proteins such as RPN1 and RPL26/uL24, promoting reticulophagy of endoplasmic reticulum sheets. Ufmylation-dependent reticulophagy inhibits the unfolded protein response (UPR) by regulating ERN1/IRE1-alpha stability. Ufmylation in response to endoplasmic reticulum stress is essential for processes such as hematopoiesis or inflammatory response. Required for TRIP4 ufmylation, thereby regulating nuclear receptors-mediated. transcription. May play a role in NF-kappa-B-mediated transcription through regulation of the phosphorylation and the degradation of NFKBIA, the inhibitor of NF-kappa-B. Plays a role in cartilage development through SOX9, inhibiting the ubiquitin-mediated proteasomal degradation of this transcriptional regulator. |
| Protein Name | Ddrgk Domain-Containing Protein 1DashurinUfm1-Binding And Pci Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-8980692 |
| Cellular Localisation | Endoplasmic ReticulumEndoplasmic Reticulum MembraneLocalizes To The Endoplasmic Reticulum Membrane In Response To Endoplasmic Reticulum Stress |
| Alternative Antibody Names | Anti-Ddrgk Domain-Containing Protein 1 antibodyAnti-Dashurin antibodyAnti-Ufm1-Binding And Pci Domain-Containing Protein 1 antibodyAnti-DDRGK1 antibodyAnti-C20orf116 antibodyAnti-UFBP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
