Anti-DDRGK1 antibody (STJA0006843)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-2000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	DDRGK1
Gene ID:	65992
Uniprot ID:	DDRGK_HUMAN
Specificity:	This antibody detects endogenous levels of DDRGK1 at Human, Mouse
Immunogen:	Synthesized peptide derived from human DDRGK1

Post Translational Modifications	Ubiquitinated. Ubiquitination probably triggers proteasomal degradation and is negatively regulated by UFL1, the enzyme involved in the ufmylation of DDRGK1. Ufmylated.conjugated to ubiquitin-like protein UFM1, probably at Lys-267 by UFL1. The relevance of ufmylation is however unclear: as DDRGK1 acts as substrate adapters for ufmylation, it is uncertain whether ufmylation is a collatoral effect of ufmylation process or is required to regulate its activity.
Function	Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, which plays a key role in reticulophagy (also called ER-phagy). In response to endoplasmic reticulum stress, promotes recruitment of the E3 UFM1-protein ligase UFL1 to the endoplasmic reticulum membrane: in turn, UFL1 mediates ufmylation of proteins such as RPN1 and RPL26/uL24, promoting reticulophagy of endoplasmic reticulum sheets. Ufmylation-dependent reticulophagy inhibits the unfolded protein response (UPR) by regulating ERN1/IRE1-alpha stability. Ufmylation in response to endoplasmic reticulum stress is essential for processes such as hematopoiesis or inflammatory response. Required for TRIP4 ufmylation, thereby regulating nuclear receptors-mediated. transcription. May play a role in NF-kappa-B-mediated transcription through regulation of the phosphorylation and the degradation of NFKBIA, the inhibitor of NF-kappa-B. Plays a role in cartilage development through SOX9, inhibiting the ubiquitin-mediated proteasomal degradation of this transcriptional regulator.
Protein Name	Ddrgk Domain-Containing Protein 1 Dashurin Ufm1-Binding And Pci Domain-Containing Protein 1
Database Links	Reactome: R-HSA-8980692
Cellular Localisation	Endoplasmic Reticulum Endoplasmic Reticulum Membrane Localizes To The Endoplasmic Reticulum Membrane In Response To Endoplasmic Reticulum Stress
Alternative Antibody Names	Anti-Ddrgk Domain-Containing Protein 1 antibody Anti-Dashurin antibody Anti-Ufm1-Binding And Pci Domain-Containing Protein 1 antibody Anti-DDRGK1 antibody Anti-C20orf116 antibody Anti-UFBP1 antibody

Information sourced from Uniprot.org

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