• Immunofluorescence analysis of NIH-3T3 cells using DDB1 Polyclonal Antibody (STJ23352) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using DDB1 Polyclonal Antibody (STJ23352) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using DDB1 Antibody (STJ23352) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using DDB1 Antibody (STJ23352) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using DDB1 Antibody (STJ23352) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using DDB1 Antibody (STJ23352) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DDB1 antibody (741-1140) (STJ23352)

SKU:
STJ23352

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDB1 (741-1140) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDB1
Gene ID: 1642
Uniprot ID: DDB1_HUMAN
Immunogen Region: 741-1140
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 741-1140 of human DDB1 (NP_001914.3).
Immunogen Sequence: EVQDTSGGTTALRPSASTQA LSSSVSSSKLFSSSTAPHET SFGEEVEVHNLLIIDQHTFE VLHAHQFLQNEYALSLVSCK LGKDPNTYFIVGTAMVYPEE AEPKQGRIVVFQYSDGKLQT VAEKEVKGAVYSMVEFNGKL LASINSTVRLYEWTTEKELR TECNHYNNIMALYLKTKGDF ILVGDLMRSVLLLAYKPMEG NFEEIARDFNPNWMSAVEIL DDDNFLGAENAFNLFVCQK
Post Translational Modifications Phosphorylated by ABL1. Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. Acetylated, promoting interaction with CUL4 (CUL4A or CUL4B) and subsequent formation of DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Deacetylation by SIRT7 impairs the interaction with CUL4 (CUL4A or CUL4B) and formation of DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes.
Function Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1. DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver. By acting on TET dioxygenses, essential for oocyte maintenance at the primordial follicle stage, hence essential for female fertility. Maternal factor required for proper zygotic genome activation and genome reprogramming.
Protein Name Dna Damage-Binding Protein 1
Ddb P127 Subunit
Dna Damage-Binding Protein A
Ddba
Damage-Specific Dna-Binding Protein 1
Hbv X-Associated Protein 1
Xap-1
Uv-Damaged Dna-Binding Factor
Uv-Damaged Dna-Binding Protein 1
Uv-Ddb 1
Xpe-Binding Factor
Xpe-Bf
Xeroderma Pigmentosum Group E-Complementing Protein
Xpce
Database Links Reactome: R-HSA-110314
Reactome: R-HSA-5696394
Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-8951664
Cellular Localisation Cytoplasm
Nucleus
Primarily Cytoplasmic
Translocates To The Nucleus Following Uv Irradiation And Subsequently Accumulates At Sites Of Dna Damage
More Concentrated In Nuclei Than In Cytoplasm In Germinal Vesicle (Gv) Stage Oocytes
Zygotes And The 2-Cell Stage
But Distributed In The Cytoplasm At The Mii-Stage Oocytes
Alternative Antibody Names Anti-Dna Damage-Binding Protein 1 antibody
Anti-Ddb P127 Subunit antibody
Anti-Dna Damage-Binding Protein A antibody
Anti-Ddba antibody
Anti-Damage-Specific Dna-Binding Protein 1 antibody
Anti-Hbv X-Associated Protein 1 antibody
Anti-Xap-1 antibody
Anti-Uv-Damaged Dna-Binding Factor antibody
Anti-Uv-Damaged Dna-Binding Protein 1 antibody
Anti-Uv-Ddb 1 antibody
Anti-Xpe-Binding Factor antibody
Anti-Xpe-Bf antibody
Anti-Xeroderma Pigmentosum Group E-Complementing Protein antibody
Anti-Xpce antibody
Anti-DDB1 antibody
Anti-XAP1 antibody

Information sourced from Uniprot.org

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