• STJ70103 (0. 3µg/ml) staining of HepG2 (A) and LNCaP (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70103 (0. 3µg/ml) staining of Human Cerebellum (A) , Kidney (B) and Testes (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70103 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic, nuclear and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70103 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70103 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-DDAH1 antibody (C-Term) (STJ70103)

SKU:
STJ70103-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-DDAH1 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in HeLa and U2OS cells. 10µg/ml
FC-Flow cytometric analysis of HeLa cells. 10ug/ml
ELISA-antibody detection limit dilution 1:1000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DDAH1
Gene ID: 23576
Uniprot ID: DDAH1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_036269.1; NP_001127917.1; NP_001317584.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).
Immunogen Sequence: TCCSVLINKKVDS
Function Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Protein Name N(G -N(G-Dimethylarginine Dimethylaminohydrolase 1
Ddah-1
Dimethylarginine Dimethylaminohydrolase 1
Ddahi
Dimethylargininase-1
Database Links Reactome: R-HSA-203615
Alternative Antibody Names Anti-N(G -N(G-Dimethylarginine Dimethylaminohydrolase 1 antibody
Anti-Ddah-1 antibody
Anti-Dimethylarginine Dimethylaminohydrolase 1 antibody
Anti-Ddahi antibody
Anti-Dimethylargininase-1 antibody
Anti-DDAH1 antibody
Anti-DDAH antibody

Information sourced from Uniprot.org

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