• Biotinylated STJ72189 (0. 5ug/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-DCUN1D1, Biotinlyated antibody (C-Term) (STJ73498)

SKU:
STJ73498-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-DCUN1D1, Biotinlyated (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DCUN1D1
Gene ID: 54165
Uniprot ID: DCNL1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_065691.2
Immunogen Sequence: RPQIAGTKSTT.
Post Translational Modifications Mono- and poly-ubiquitinated by ARIH2 and ARIH1. Monoubiquitination by ARIH2 is mediated by an interaction between autoubiquitinated ARIH2 and the UBA-like domain. The monoubiquitinated form preferentially interacts with non-neddylated cullins and modulates cullin RING ligase (CRL) complex composition and activity.
Function Part of an E3 ubiquitin ligase complex for neddylation. Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression.
Protein Name Dcn1-Like Protein 1
Dcnl1
Dcun1 Domain-Containing Protein 1
Defective In Cullin Neddylation Protein 1-Like Protein 1
Squamous Cell Carcinoma-Related Oncogene
Database Links Reactome: R-HSA-8951664
Cellular Localisation Nucleus
Cytoplasm
The Ubiquitinated Form Is Localized In The Cytoplasm
Alternative Antibody Names Anti-Dcn1-Like Protein 1 antibody
Anti-Dcnl1 antibody
Anti-Dcun1 Domain-Containing Protein 1 antibody
Anti-Defective In Cullin Neddylation Protein 1-Like Protein 1 antibody
Anti-Squamous Cell Carcinoma-Related Oncogene antibody
Anti-DCUN1D1 antibody
Anti-DCN1 antibody
Anti-DCUN1L1 antibody
Anti-RP42 antibody
Anti-SCCRO antibody

Information sourced from Uniprot.org

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