• STJ72400 (0. 03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ72400 (0. 2µg/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ72400 (8µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ72400 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Anti-DCTN3 antibody (Internal) (STJ72400)

SKU:
STJ72400-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-DCTN3 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DCTN3
Gene ID: 11258
Uniprot ID: DCTN3_HUMAN
Immunogen Region: Internal
Accession Number: NP_009165.1; NP_077324.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).
Immunogen Sequence: QVALGNISSKRE
Function Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. Together with dynein may be involved in spindle assembly and cytokinesis.
Protein Name Dynactin Subunit 3
Dynactin Complex Subunit 22 Kda Subunit
P22
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-2565942
Reactome: R-HSA-3371497
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-6807878
Reactome: R-HSA-6811436
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Chromosome
Centromere
Kinetochore
Spindle
Cleavage Furrow
Midbody
Localizes To Punctate Cytoplasmic Structures And To The Centrosome During Interphase
And To Kinetochores And To Spindle Poles Throughout Mitosis
Colocalizes With Dynein To The Cleavage Furrow And To Midbody Of Dividing Cells
Alternative Antibody Names Anti-Dynactin Subunit 3 antibody
Anti-Dynactin Complex Subunit 22 Kda Subunit antibody
Anti-P22 antibody
Anti-DCTN3 antibody
Anti-DCTN22 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance