• Immunohistochemistry analysis of paraffin-embedded mouse kidney using DCTD antibody (STJ28162) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using DCTD antibody (STJ28162) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using DCTD antibody (STJ28162) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DCTD antibody (1-178) (STJ28162)

SKU:
STJ28162

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DCTD
Gene ID: 1635
Uniprot ID: DCTD_HUMAN
Immunogen Region: 1-178
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human DCTD (NP_001912.2).
Immunogen Sequence: MSEVSCKKRDDYLEWPEYFM AVAFLSAQRSKDPNSQVGAC IVNSENKIVGIGYNGMPNGC SDDVLPWRRTAENKLDTKYP YVCHAELNAIMNKNSTDVKG CSMYVALFPCNECAKLIIQA GIKEVIFMSDKYHDSDEATA ARLLFNMAGVTFRKFIPKCS KIVIDFDSINSRPSQKLQ
Function Catalyzes the deamination of dCMP to dUMP, providing the nucleoside monophosphate substrate for the thymidylate synthase/TYMS. Also, part of a nucleotide salvage pathway that eliminates epigenetically modified 5-hydroxymethyl-dCMP (hmdCMP) in a two-step process entailing deamination to cytotoxic 5-hydroxymethyl-dUMP (hmdUMP), followed by its hydrolysis into 5-hydroxymethyluracil (hmU) and 2-deoxy-D-ribose 5-phosphate (deoxyribosephosphate). Catalyzes the first step in that pathway, the deamination of 5-hydroxymethyl-dCMP (hmdCMP).
Protein Name Deoxycytidylate Deaminase
Dcmp Deaminase
Database Links Reactome: R-HSA-499943
Alternative Antibody Names Anti-Deoxycytidylate Deaminase antibody
Anti-Dcmp Deaminase antibody
Anti-DCTD antibody

Information sourced from Uniprot.org

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