• Western blot analysis of various lysates, using DCAMKL1/DCLK1 antibody (STJ113645) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of Rat brain using DCAMKL1/DCLK1 antibody (STJ113645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain using DCAMKL1/DCLK1 antibody (STJ113645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat brain cells using DCAMKL1/DCLK1 rabbit polyclonal antibody (STJ113645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain cells using DCAMKL1/DCLK1 rabbit polyclonal antibody (STJ113645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DCLK1 antibody (590-740) (STJ113645)

SKU:
STJ113645

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DCLK1 (590-740) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DCLK1
Gene ID: 9201
Uniprot ID: DCLK1_HUMAN
Immunogen Region: 590-740
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 590-740 of DCAMKL1/DCLK1 (NP_001317000.1).
Immunogen Sequence: SGDDQEVLFDQILMGQVDFP SPYWDNVSDSAKELITMMLL VDVDQRFSAVQVLEHPWVND DGLPENEHQLSVAGKIKKHF NTGPKPNSTAAGVSVIATTA LDKERQVFRRRRNQDVRSRY KAQPAPPELNSESEDYSPSS SETVRSPNSPF
Tissue Specificity In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
Function Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
Protein Name Serine/Threonine-Protein Kinase Dclk1
Doublecortin Domain-Containing Protein 3a
Doublecortin-Like And Cam Kinase-Like 1
Doublecortin-Like Kinase 1
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Dclk1 antibody
Anti-Doublecortin Domain-Containing Protein 3a antibody
Anti-Doublecortin-Like And Cam Kinase-Like 1 antibody
Anti-Doublecortin-Like Kinase 1 antibody
Anti-DCLK1 antibody
Anti-DCAMKL1 antibody
Anti-DCDC3A antibody
Anti-KIAA0369 antibody

Information sourced from Uniprot.org

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