• Western blot analysis of HeLa, using DCD antibody (STJ29419) at 1:8000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of 293T, using DCD antibody (STJ29419) at 1:8000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DCD antibody (20-110) (STJ29419)

SKU:
STJ29419

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DCD (20-110) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:9000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DCD
Gene ID: 117159
Uniprot ID: DCD_HUMAN
Immunogen Region: 20-110
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human DCD (NP_444513.1).
Immunogen Sequence: YDPEAASAPGSGNPCHEASA AQKENAGEDPGLARQAPKPR KQRSSLLEKGLDGAKKAVGG LGKLGKDAVEDLESVGKGAV HDVKDVLDSVL
Tissue Specificity Detected in urine (at protein level). Constitutively expressed in eccrine sweat gland cells (at protein level). Secreted into the sweat at a concentration of 1-10 micrograms/ml.
Function DCD-1: Found in sweat, has an antimicrobial activity during early bacterial colonization. The secreted peptide assembles into homohexameric complexes that can associate with and also insert into pathogen membranes. Once inserted in bacteria membranes forms anion channels probably altering the transmembrane potential essential for bacterial survival. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity, cleaving on the C-terminal side of Arg and, to a lesser extent, Lys residues. Survival-promoting peptide: Promotes survival of neurons and displays phosphatase activity. It may bind IgG.
Protein Name Dermcidin
Preproteolysin Cleaved Into - Survival-Promoting Peptide - Dcd-1
Database Links Reactome: R-HSA-6803157
Cellular Localisation Secreted
Survival-Promoting Peptide: Secreted
Dcd-1: Secreted
Membrane
Peripheral Membrane Protein
Single-Pass Membrane Protein
The Secreted Peptide Assembles Into Homohexameric Complexes That Can Probably Associate With Pathogen Membranes And Also Insert Into These Membranes Where They Behave As Channels
Alternative Antibody Names Anti-Dermcidin antibody
Anti-Preproteolysin Cleaved Into - Survival-Promoting Peptide - Dcd-1 antibody
Anti-DCD antibody
Anti-AIDD antibody
Anti-DSEP antibody

Information sourced from Uniprot.org

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