• STJ70171 (0. 3µg/ml) staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.
  • STJ70171 staining (0. 3µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.
  • STJ70171 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70171 (1µg/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35µg protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence. This data is from a previous batch, not on sale.

Anti-DAP3 antibody (C-Term) (STJ70171)

SKU:
STJ70171-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-DAP3 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-1µg/ml
IF-Strong expression of the protein seen in the nuclei of MCF7 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DAP3
Gene ID: 7818
Uniprot ID: RT29_HUMAN
Immunogen Region: C-Term
Accession Number: NP_387506.1; NP_001186779.1; NP_001186780.1
Specificity: This antibody is expected to recognise isoform 1 (NP_387506.1) , isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1).
Immunogen Sequence: NPSLLERHCAYL
Function Involved in mediating interferon-gamma-induced cell death.
Protein Name Small Ribosomal Subunit Protein Ms29
28s Ribosomal Protein S29 - Mitochondrial
Mrp-S29
S29mt
Death-Associated Protein 3
Dap-3
Ionizing Radiation Resistance Conferring Protein
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Ms29 antibody
Anti-28s Ribosomal Protein S29 - Mitochondrial antibody
Anti-Mrp-S29 antibody
Anti-S29mt antibody
Anti-Death-Associated Protein 3 antibody
Anti-Dap-3 antibody
Anti-Ionizing Radiation Resistance Conferring Protein antibody
Anti-DAP3 antibody
Anti-MRPS29 antibody

Information sourced from Uniprot.org

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