• Western blot analysis of extracts of Rat kidney, using DAO antibody (STJ27262) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Confocal immunofluorescence analysis of L929 cells using DAO Polyclonal Antibody (STJ27262) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat kidney cells using DAO rabbit polyclonal antibody (STJ27262) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-DAO antibody (1-347) (STJ27262)

SKU:
STJ27262

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DAO (1-347) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DAO
Gene ID: 1610
Uniprot ID: OXDA_HUMAN
Immunogen Region: 1-347
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-347 of human DAO (NP_001908.3).
Immunogen Sequence: MRVVVIGAGVIGLSTALCIH ERYHSVLQPLDIKVYADRFT PLTTTDVAAGLWQPYLSDPN NPQEADWSQQTFDYLLSHVH SPNAENLGLFLISGYNLFHE AIPDPSWKDTVLGFRKLTPR ELDMFPDYGYGWFHTSLILE GKNYLQWLTERLTERGVKFF QRKVESFEEVAREGADVIVN CTGVWAGALQRDPLLQPGRG QIMKVDAPWMKHFILTHDPE RGIYNSPYIIPGTQTVTLG
Function Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
Protein Name D-Amino-Acid Oxidase
Daao
Damox
Dao
Database Links Reactome: R-HSA-389661
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-D-Amino-Acid Oxidase antibody
Anti-Daao antibody
Anti-Damox antibody
Anti-Dao antibody
Anti-DAO antibody
Anti-DAMOX antibody

Information sourced from Uniprot.org

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