Anti-DAGLA antibody (C-Term) (STJ71663)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-DAGLA (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 125kDa band observed in Human Liver lysates (calculated MW of 115kDa according to NP_006124.1). Recommended concentration: 0.3-1µg/ml. Some minor background is detected and is |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | DAGLA |
| Gene ID | 747 |
| Uniprot ID | DGLA_HUMAN |
| Accession Number | NP_006124.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | PAKQDELVISAR |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-782 and Ser-808 by CAMK2A.phosphorylation by CAMK2A inhibits diacylglycerol lipase activity. |
| Function | Serine hydrolase that hydrolyzes arachidonic acid-esterified diacylglycerols (DAGs) to produce the principal endocannabinoid, 2-arachidonoylglycerol (2-AG). Preferentially hydrolyzes sn-1 fatty acids from diacylglycerols (DAG) that contain arachidonic acid (AA) esterified at the sn-2 position to biosynthesize 2-AG. Has negligible activity against other lipids including monoacylglycerols and phospholipids. Plays a key role in regulating 2-AG signaling in the central nervous system (CNS). Regulates 2-AG involved in retrograde suppression at central synapses. Supports axonal growth during development and adult neurogenesis. Plays a role for eCB signaling in the physiological regulation of anxiety and depressive behaviors. Regulates also neuroinflammatory responses in the brain, in particular, LPS-induced microglial activation. |
| Protein Name | Diacylglycerol Lipase-AlphaDagl-AlphaDgl-AlphaNeural Stem Cell-Derived Dendrite RegulatorSn1-Specific Diacylglycerol Lipase Alpha |
| Database Links | Reactome: R-HSA-426048 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinPostsynaptic Density MembraneEarly Endosome MembraneCell ProjectionDendritic Spine MembraneCycles Between The Cell Surface And An Intracellular Endosomal CompartmentInternalized By Early Endosomes Via A Clathrin-Independent Pathway Before Transport Back To The Postsynaptic Membrane Surface In A Pkc-Dependent Manner |
| Alternative Antibody Names | Anti-Diacylglycerol Lipase-Alpha antibodyAnti-Dagl-Alpha antibodyAnti-Dgl-Alpha antibodyAnti-Neural Stem Cell-Derived Dendrite Regulator antibodyAnti-Sn1-Specific Diacylglycerol Lipase Alpha antibodyAnti-DAGLA antibodyAnti-C11orf11 antibodyAnti-KIAA0659 antibodyAnti-NSDDR antibody |
Information sourced from Uniprot.org