Anti-Cytokeratin 5 antibody (318-491aa) [ZM186] (STJ180376)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenRecombinant human Cytokeratin 5 (KRT5) protein fragment (around aa 318-491)
General Information
| Short Description | Mouse monoclonal Cytokeratin 5 (318-491aa) antibody for use in IHC-P in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ZM186 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Purification | Affinity purified |
| Dilution Range | 1:100‐200 |
| Formulation | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Storage Instruction | Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
Target Information
| Gene Symbol | KRT5 |
| Gene ID | 3852 |
| Uniprot ID | K2C5_HUMAN |
| Immunogen | Recombinant human Cytokeratin 5 (KRT5) protein fragment (around aa 318-491) |
| Immunogen Region | 318-491aa |
| Specificity | Positive control: Prostate |
Additional Info
| Tissue Specificity | Expressed in corneal epithelium (at protein level). Expressed in keratinocytes (at protein level). |
| Post Translational Modifications | Phosphorylated by CDK1, AURKB and Rho-kinase, phosphorylation is regulated by the cell cycle. Thr-24 phosphorylation, mediated by CDK1, peaks during prometaphase or metaphase cells with phosphorylated filamentous structures evident throughout the cytoplasm during early mitosis. CDK1 phosphorylates Thr-24 in mitotic cells at the site of injury. O-glycosylated. |
| Function | Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress. Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes. |
| Protein Name | Keratin - Type Ii Cytoskeletal 558 Kda CytokeratinCytokeratin-5Ck-5Keratin-5K5Type-Ii Keratin Kb5 |
| Database Links | Reactome: R-HSA-446107Reactome: R-HSA-6805567Reactome: R-HSA-6809371Reactome: R-HSA-9725554 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Keratin - Type Ii Cytoskeletal 5 antibodyAnti-58 Kda Cytokeratin antibodyAnti-Cytokeratin-5 antibodyAnti-Ck-5 antibodyAnti-Keratin-5 antibodyAnti-K5 antibodyAnti-Type-Ii Keratin Kb5 antibodyAnti-KRT5 antibody |
Information sourced from Uniprot.org