• Confocal imaging of paraffin-embedded Rat skin using Cytokeratin 10 Rabbit monoclonal antibody (STJ11103574, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Mouse skin using Cytokeratin 10 Rabbit monoclonal antibody (STJ11103574, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Human skin using Cytokeratin 10 Rabbit monoclonal antibody (STJ11103574, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using Cytokeratin 10 Rabbit monoclonal antibody (STJ11103574) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Cytokeratin 10 antibody (485-584) [S4MR] (STJ11103574)

SKU:
STJ11103574

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytokeratin 10 (485-584) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT10
Gene ID: 3858
Uniprot ID: K1C10_HUMAN
Immunogen Region: 485-584
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 485-584 of human Cytokeratin 10 (P13645).
Immunogen Sequence: GGHGGGHGGSSGGGYGGGSS GGGSSGGGYGGGSSSGGHGG SSSGGYGGGSSGGGGGGYGG GSSGGGSSSGGGYGGGSSSG GHKSSSSGSVGESSSKGPRY
Tissue Specificity Seen in all suprabasal cell layers including stratum corneum. Expressed on the surface of lung cell lines. Localized on the surface of desquamated nasal epithelial cells (at protein level).
Function Plays a role in the establishment of the epidermal barrier on plantar skin. Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium. (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.
Protein Name Keratin - Type I Cytoskeletal 10
Cytokeratin-10
Ck-10
Keratin-10
K10
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Secreted
Extracellular Space
Cell Surface
Cytoplasm
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 10 antibody
Anti-Cytokeratin-10 antibody
Anti-Ck-10 antibody
Anti-Keratin-10 antibody
Anti-K10 antibody
Anti-KRT10 antibody
Anti-KPP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance