Anti-CYP7B1 antibody (C-Term) (STJ70464)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-CYP7B1 (C-Term) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IHC |
| Host | Goat |
| Reactivity | Human/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments in Human Liver, Kidney and Prostate lysates gave no specific signal but low background (at antibody concentration up to 1ยตg/ml). We would appreciate any feedba |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP7B1 |
| Gene ID | 9420 |
| Uniprot ID | CP7B1_HUMAN |
| Accession Number | NP_004811.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | YPDSDVLFRYKVKS |
Additional Info
| Function | A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position. Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase. Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids. Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen-specific humoral immune response. 7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning. Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives. |
| Protein Name | Cytochrome P450 7b124-Hydroxycholesterol 7-Alpha-Hydroxylase25/26-Hydroxycholesterol 7-Alpha-Hydroxylase3-Hydroxysteroid 7-Alpha HydroxylaseOxysterol 7-Alpha-Hydroxylase |
| Database Links | Reactome: R-HSA-192105Reactome: R-HSA-193368Reactome: R-HSA-193807Reactome: R-HSA-211976Reactome: R-HSA-5579013 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-Cytochrome P450 7b1 antibodyAnti-24-Hydroxycholesterol 7-Alpha-Hydroxylase antibodyAnti-25/26-Hydroxycholesterol 7-Alpha-Hydroxylase antibodyAnti-3-Hydroxysteroid 7-Alpha Hydroxylase antibodyAnti-Oxysterol 7-Alpha-Hydroxylase antibodyAnti-CYP7B1 antibody |
Information sourced from Uniprot.org