| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CYP7B1 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CYP7B1 |
| Gene ID: |
9420 |
| Uniprot ID: |
CP7B1_HUMAN |
| Immunogen Region: |
1-100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CYP7B1 (NP_004811.1). |
| Immunogen Sequence: |
MAGEVSAATGRFSLERLGLP GLALAAALLLLALCLLVRRT RRPGEPPLIKGWLPYLGVVL NLRKDPLRFMKTLQKQHGDT FTVLLGGKYITFILDPFQYQ |
| Tissue Specificity | Widely expressed. Expressed in brain, testis, ovary, prostate, liver, colon, kidney, small intestine, thymus and spleen. |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position. Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase. Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids. Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen-specific humoral immune response. 7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning. Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives. |
| Protein Name | Cytochrome P450 7b124-Hydroxycholesterol 7-Alpha-Hydroxylase25/26-Hydroxycholesterol 7-Alpha-Hydroxylase3-Hydroxysteroid 7-Alpha HydroxylaseOxysterol 7-Alpha-Hydroxylase |
| Database Links | Reactome: R-HSA-192105Reactome: R-HSA-193368Reactome: R-HSA-193807Reactome: R-HSA-211976Reactome: R-HSA-5579013 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-Cytochrome P450 7b1 antibodyAnti-24-Hydroxycholesterol 7-Alpha-Hydroxylase antibodyAnti-25/26-Hydroxycholesterol 7-Alpha-Hydroxylase antibodyAnti-3-Hydroxysteroid 7-Alpha Hydroxylase antibodyAnti-Oxysterol 7-Alpha-Hydroxylase antibodyAnti-CYP7B1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
