Anti-CYP39A1 antibody (300-400 aa) (STJ11105028)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal 24-Hydroxycholesterol 7-Alpha-Hydroxylase (300-400 aa) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP39A1 |
| Gene ID | 51302 |
| Uniprot ID | CP39A_HUMAN |
| Immunogen Region | 300-400 aa |
| Immunogen Sequence | MEGISSVFGKAGKDKIKVSE DDLENLLLIKWCVLETIRLK APGVITRKVVKPVEILNYII PSGDLLMLSPFWLHRNPKYF PEPELFKPERWKKANLEKHS F |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human CYP39A1. (NP_057677.2). |
Additional Info
| Tissue Specificity | Liver specific. |
| Function | A cytochrome P450 monooxygenase involved in neural cholesterol clearance through bile acid synthesis. Catalyzes 7-alpha hydroxylation of (24S)-hydroxycholesterol, a neural oxysterol that is metabolized to bile acids in the liver. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). |
| Protein Name | 24-Hydroxycholesterol 7-Alpha-HydroxylaseCytochrome P450 39a1Hcyp39a1Oxysterol 7-Alpha-Hydroxylase |
| Database Links | Reactome: R-HSA-193775Reactome: R-HSA-211976 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-24-Hydroxycholesterol 7-Alpha-Hydroxylase antibodyAnti-Cytochrome P450 39a1 antibodyAnti-Hcyp39a1 antibodyAnti-Oxysterol 7-Alpha-Hydroxylase antibodyAnti-CYP39A1 antibody |
Information sourced from Uniprot.org