Anti-CYP11B2 antibody (1-100) (STJ11105453)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Cytochrome P450 11b2-Mitochondrial (1-100) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IF/ICC:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP11B2 |
| Gene ID | 1585 |
| Uniprot ID | C11B2_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MALRAKAEVCVAAPWLSLQR ARALGTRAARAPRTVLPFEA MPQHPGNRWLRLLQIWREQG YEHLHLEMHQTFQELGPIFR YNLGGPRMVCVMLPEDVEKL |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CYP11B2 (NP_000489.3). |
Additional Info
| Tissue Specificity | Expressed sporadically in the zona glomerulosa (zG) of the adrenal cortex (conventional zonation), as well as in aldosterone-producing cell clusters (APCCs) composed of morphological zG cells in contact with the capsule (variegated zonation). |
| Function | A cytochrome P450 monooxygenase that catalyzes the biosynthesis of aldosterone, the main mineralocorticoid in the human body responsible for salt and water homeostasis, thus involved in blood pressure regulation, arterial hypertension, and the development of heart failure. Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone (21-hydroxyprogesterone), namely 11-beta hydroxylation, followed by two successive oxidations at C18 yielding 18-hydroxy and then 18-oxo intermediates (that would not leave the enzyme active site during the consecutive hydroxylation reactions), ending with the formation of aldosterone. Can also produce 18-hydroxycortisol and 18-oxocortisol, derived from successive oxidations of cortisol at C18, normally found at very low levels, but significantly increased in primary aldosteronism, the most common form of secondary hypertension. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Could also be involved in the androgen metabolic pathway (Probable). |
| Protein Name | Cytochrome P450 11b2 - MitochondrialAldosterone SynthaseAldosAldosterone-Synthesizing EnzymeCypxib2Corticosterone 18-Monooxygenase - Cyp11b2Cytochrome P-450aldoCytochrome P-450c18Steroid 11-Beta-Hydroxylase - Cyp11b2Steroid 18-Hydroxylase |
| Database Links | Reactome: R-HSA-193993Reactome: R-HSA-194002Reactome: R-HSA-211976Reactome: R-HSA-5579009 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Cytochrome P450 11b2 - Mitochondrial antibodyAnti-Aldosterone Synthase antibodyAnti-Aldos antibodyAnti-Aldosterone-Synthesizing Enzyme antibodyAnti-Cypxib2 antibodyAnti-Corticosterone 18-Monooxygenase - Cyp11b2 antibodyAnti-Cytochrome P-450aldo antibodyAnti-Cytochrome P-450c18 antibodyAnti-Steroid 11-Beta-Hydroxylase - Cyp11b2 antibodyAnti-Steroid 18-Hydroxylase antibodyAnti-CYP11B2 antibody |
Information sourced from Uniprot.org