Anti-CYP11B1 antibody (234-503) (STJ117240)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CYP11B1 (234-503) antibody for use in IHC-P, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IHC-P:1:100-1:500IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP11B1 |
| Gene ID | 1584 |
| Uniprot ID | C11B1_HUMAN |
| Immunogen Region | 234-503 |
| Immunogen Sequence | TVQLMFMPRSLSRWTSPKVW KEHFEAWDCIFQYGDNCIQK IYQELAFSRPQQYTSIVAEL LLNAELSPDAIKANSMELTA GSVDTTVFPLLMTLFELARN PNVQQALRQESLAAAASISE HPQKATTELPLLRAALKETL RLYPVGLFLERVASSDLVLQ NYHIPAGTLVRVFLYSLGRN PALFPRPERYNPQRWLDIRG SGRNFYHVPFGFGMRQCLGR RLAEAEMLLLLHHVLKHLQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human CYP11B1 (NP_000488.3). |
Additional Info
| Tissue Specificity | Expressed in the zona fasciculata/reticularis of the adrenal cortex. |
| Function | A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones. Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Due to its lack of 18-oxidation activity, it is incapable of generating aldosterone. Could also be involved in the androgen metabolic pathway (Probable). |
| Protein Name | Cytochrome P450 11b1 - MitochondrialCyp11b1Cypxib1Cytochrome P-450c11Cytochrome P450c11Steroid 11-Beta-Hydroxylase - Cyp11b1 |
| Database Links | Reactome: R-HSA-194002Reactome: R-HSA-211976Reactome: R-HSA-5579017 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Cytochrome P450 11b1 - Mitochondrial antibodyAnti-Cyp11b1 antibodyAnti-Cypxib1 antibodyAnti-Cytochrome P-450c11 antibodyAnti-Cytochrome P450c11 antibodyAnti-Steroid 11-Beta-Hydroxylase - Cyp11b1 antibodyAnti-CYP11B1 antibodyAnti-S11BH antibody |
Information sourced from Uniprot.org