| Host: |
Rabbit |
| Applications: |
IHC/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CYP11B1 (234-503) is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC-P 1:100-1:500IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CYP11B1 |
| Gene ID: |
1584 |
| Uniprot ID: |
C11B1_HUMAN |
| Immunogen Region: |
234-503 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human CYP11B1 (NP_000488.3). |
| Immunogen Sequence: |
TVQLMFMPRSLSRWTSPKVW KEHFEAWDCIFQYGDNCIQK IYQELAFSRPQQYTSIVAEL LLNAELSPDAIKANSMELTA GSVDTTVFPLLMTLFELARN PNVQQALRQESLAAAASISE HPQKATTELPLLRAALKETL RLYPVGLFLERVASSDLVLQ NYHIPAGTLVRVFLYSLGRN PALFPRPERYNPQRWLDIRG SGRNFYHVPFGFGMRQCLGR RLAEAEMLLLLHHVLKHLQ |
| Tissue Specificity | Expressed in the zona fasciculata/reticularis of the adrenal cortex. |
| Function | A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones. Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Due to its lack of 18-oxidation activity, it is incapable of generating aldosterone. Could also be involved in the androgen metabolic pathway (Probable). |
| Protein Name | Cytochrome P450 11b1 - MitochondrialCyp11b1Cypxib1Cytochrome P-450c11Cytochrome P450c11Steroid 11-Beta-Hydroxylase - Cyp11b1 |
| Database Links | Reactome: R-HSA-194002Reactome: R-HSA-211976Reactome: R-HSA-5579017 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Cytochrome P450 11b1 - Mitochondrial antibodyAnti-Cyp11b1 antibodyAnti-Cypxib1 antibodyAnti-Cytochrome P-450c11 antibodyAnti-Cytochrome P450c11 antibodyAnti-Steroid 11-Beta-Hydroxylase - Cyp11b1 antibodyAnti-CYP11B1 antibodyAnti-S11BH antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
