Anti-CYP11B1 antibody (61-110 aa) (STJ96589)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the N-terminal region of human CYP11B1/2 at the amino acid range 61-110
General Information
| Short Description | Rabbit polyclonal anti-Cytochrome P450 11B1, mitochondrial (61-110 aa) for use in WB and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP11B1 |
| Gene ID | 1584 |
| Uniprot ID | C11B1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human CYP11B1/2 at the amino acid range 61-110 |
| Immunogen Region | 61-110 aa |
| Specificity | CYP11B1/2 Polyclonal Antibody detects endogenous levels of CYP11B1/2 protein. |
Additional Info
| Function | A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones. Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Due to its lack of 18-oxidation activity, it is incapable of generating aldosterone. Could also be involved in the androgen metabolic pathway (Probable). |
| Protein Name | Cytochrome P450 11b1 - MitochondrialCyp11b1Cypxib1Cytochrome P-450c11Cytochrome P450c11Steroid 11-Beta-Hydroxylase - Cyp11b1 |
| Database Links | Reactome: R-HSA-194002Reactome: R-HSA-211976Reactome: R-HSA-5579017 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Cytochrome P450 11b1 - Mitochondrial antibodyAnti-Cyp11b1 antibodyAnti-Cypxib1 antibodyAnti-Cytochrome P-450c11 antibodyAnti-Cytochrome P450c11 antibodyAnti-Steroid 11-Beta-Hydroxylase - Cyp11b1 antibodyAnti-CYP11B1 antibodyAnti-S11BH antibody |
Information sourced from Uniprot.org