• Immunofluorescence analysis of NIH/3T3 cells using Cytochrome C Rabbit polyclonal antibody (STJ23308) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Cytochrome C in paraffin-embedded human oophoroma using Cytochrome C Rabbit polyclonal antibody (STJ23308) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Cytochrome C in paraffin-embedded human breast cancer using Cytochrome C Rabbit polyclonal antibody (STJ23308) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Cytochrome C Rabbit polyclonal antibody (STJ23308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CYCS antibody (1-105) (STJ23308)

SKU:
STJ23308

€161.88 - €799.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cytochrome c (1-105) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYCS
Gene ID: 54205
Uniprot ID: CYC_HUMAN
Immunogen Region: 1-105
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome C (NP_061820.1).
Immunogen Sequence: MGDVEKGKKIFIMKCSQCHT VEKGGKHKTGPNLHGLFGRK TGQAPGYSYTAANKNKGIIW GEDTLMEYLENPKKYIPGTK MIFVGIKKKEERADLIAYLK KATNE
Post Translational Modifications Binds 1 heme c group covalently per subunit. Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.
Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Protein Name Cytochrome C
Database Links Reactome: R-HSA-111457
Reactome: R-HSA-111458
Reactome: R-HSA-111459
Reactome: R-HSA-111463
Reactome: R-HSA-111464
Reactome: R-HSA-2151201
Reactome: R-HSA-3299685
Reactome: R-HSA-5620971
Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9627069
Reactome: R-HSA-9707564
Cellular Localisation Mitochondrion Intermembrane Space
Loosely Associated With The Inner Membrane
Alternative Antibody Names Anti-Cytochrome C antibody
Anti-CYCS antibody
Anti-CYC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance