• Western blot analysis of extracts of various cell lines, using NOX2/gp91phox antibody (STJ23306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry of paraffin-embedded rat bone marrow using NOX2/gp91phox antibody (STJ23306) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CYBB antibody (385-570) (STJ23306)

SKU:
STJ23306

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NOX2/gp91phox (385-570) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYBB
Gene ID: 1536
Uniprot ID: CY24B_HUMAN
Immunogen Region: 385-570
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 385-570 of human NOX2/gp91phox (NP_000388.2).
Immunogen Sequence: IAVDGPFGTASEDVFSYEVV MLVGAGIGVTPFASILKSVW YKYCNNATNLKLKKIYFYWL CRDTHAFEWFADLLQLLESQ MQERNNAGFLSYNIYLTGWD ESQANHFAVHHDEEKDVITG LKQKTLYGRPNWDNEFKTIA SQHPNTRIGVFLCGPEALAE TLSKQSISNSESGPRGVHFI FNKENF
Tissue Specificity Detected in neutrophils (at protein level).
Post Translational Modifications Glycosylated. Phosphorylated on Ser and Thr residues. Undergoes 'Lys-48'-linked polyubiquitination, likely by RNF145, triggering endoplasmic reticulum-associated degradation.
Function Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Protein Name Cytochrome B-245 Heavy Chain
Cgd91-Phox
Cytochrome B(558 Subunit Beta
Cytochrome B558 Subunit Beta
Heme-Binding Membrane Glycoprotein Gp91phox
Nadph Oxidase 2
Neutrophil Cytochrome B 91 Kda Polypeptide
Superoxide-Generating Nadph Oxidase Heavy Chain Subunit
Gp91-1
Gp91-Phox
P22 Phagocyte B-Cytochrome
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1236973
Reactome: R-HSA-3299685
Reactome: R-HSA-4420097
Reactome: R-HSA-5668599
Reactome: R-HSA-6798695
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
As Unassembled Monomer May Localize To The Endoplasmic Reticulum
Alternative Antibody Names Anti-Cytochrome B-245 Heavy Chain antibody
Anti-Cgd91-Phox antibody
Anti-Cytochrome B(558 Subunit Beta antibody
Anti-Cytochrome B558 Subunit Beta antibody
Anti-Heme-Binding Membrane Glycoprotein Gp91phox antibody
Anti-Nadph Oxidase 2 antibody
Anti-Neutrophil Cytochrome B 91 Kda Polypeptide antibody
Anti-Superoxide-Generating Nadph Oxidase Heavy Chain Subunit antibody
Anti-Gp91-1 antibody
Anti-Gp91-Phox antibody
Anti-P22 Phagocyte B-Cytochrome antibody
Anti-CYBB antibody
Anti-NOX2 antibody

Information sourced from Uniprot.org

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