• Immunofluorescence analysis of C6 cells using CYB5A Polyclonal Antibody (STJ27354) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using CYB5A antibody (STJ27354) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using CYB5A antibody (STJ27354) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CYB5A antibody (STJ27354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-CYB5A antibody (1-100) (STJ27354)

SKU:
STJ27354

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CYB5A (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYB5A
Gene ID: 1528
Uniprot ID: CYB5_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CYB5A (NP_683725.1).
Immunogen Sequence: MAEQSDEAVKYYTLEEIQKH NHSKSTWLILHHKVYDLTKF LEEHPGGEEVLREQAGGDAT ENFEDVGHSTDAREMSKTFI IGELHPDDRPKLNKPPETLI
Function Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.
Protein Name Cytochrome B5
Microsomal Cytochrome B5 Type A
Mcb5
Database Links Reactome: R-HSA-196836
Reactome: R-HSA-9609523 P00167-1
Cellular Localisation Isoform 1: Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Cytoplasmic Side
Microsome Membrane
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Cytochrome B5 antibody
Anti-Microsomal Cytochrome B5 Type A antibody
Anti-Mcb5 antibody
Anti-CYB5A antibody
Anti-CYB5 antibody

Information sourced from Uniprot.org

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