• Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells using 3 Mu g CXXC1 antibody (STJ115384). Western blot was performed from the immunoprecipitate using CXXC1 antibody (STJ115384) at a dilution of 1:500.
  • Western blot analysis of various lysates using CXXC1 Rabbit polyclonal antibody (STJ115384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CXXC1 antibody (1-210) (STJ115384)

SKU:
STJ115384

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Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CXXC1
Gene ID: 30827
Uniprot ID: CXXC1_HUMAN
Immunogen Region: 1-210
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human CXXC1 (NP_001095124.1).
Immunogen Sequence: MEGDGSDPEPPDAGEDSKSE NGENAPIYCICRKPDINCFM IGCDNCNEWFHGDCIRITEK MAKAIREWYCRECREKDPKL EIRYRHKKSRERDGNERDSS EPRDEGGGRKRPVPDPDLQR RAGSGTGVGAMLARGSASPH KSSPQPLVATPSQHHQQQQQ QIKRSARMCGECEACRRTED CGHCDFCRDMKKFGGPNKIR QKCRLRQCQL
Tissue Specificity Ubiquitous.
Post Translational Modifications May be regulated by proteolysis.
Function Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG.
Protein Name Cxxc-Type Zinc Finger Protein 1
Cpg-Binding Protein
Phd Finger And Cxxc Domain-Containing Protein 1
Database Links Reactome: R-HSA-381038
Reactome: R-HSA-9772755
Cellular Localisation Nucleus Speckle
Nucleus
Associated With Euchromatin
During Mitosis
Excluded From Condensed Chromosomes
Alternative Antibody Names Anti-Cxxc-Type Zinc Finger Protein 1 antibody
Anti-Cpg-Binding Protein antibody
Anti-Phd Finger And Cxxc Domain-Containing Protein 1 antibody
Anti-CXXC1 antibody
Anti-CFP1 antibody
Anti-CGBP antibody
Anti-PCCX1 antibody
Anti-PHF18 antibody

Information sourced from Uniprot.org

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