| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CXCL8 |
| Gene ID: | 3576 |
| Uniprot ID: | IL8_HUMAN |
| Immunogen Region: | 28-99 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-99 of human IL8 (NP_000575.1). |
| Immunogen Sequence: | SAKELRCQCIKTYSKPFHPK FIKELRVIESGPHCANTEII VKLSDGRELCLDPKENWVQR VVEKFLKRAENS |
| Post Translational Modifications | Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation. (Microbial infection) Cleaved by group A Streptococcus protease SpyCEP.leading to impaired neutrophil endothelial transmigration and thus increased virulence. |
| Function | Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways. |
| Protein Name | Interleukin-8 Il-8 C-X-C Motif Chemokine 8 Chemokine C-X-C Motif Ligand 8 Emoctakin Granulocyte Chemotactic Protein 1 Gcp-1 Monocyte-Derived Neutrophil Chemotactic Factor Mdncf Monocyte-Derived Neutrophil-Activating Peptide Monap Neutrophil-Activating Protein 1 Nap-1 Protein 3-10c T-Cell Chemotactic Factor Cleaved Into - Mdncf-A Gcp/Il-8 Protein Iv Il8/Nap1 Form I - Interleukin-8 (Ala-Il-877 Gcp/Il-8 Protein Ii Il-8(1-77 Il8/Nap1 Form Ii Mdncf-B - Il-8(5-77 - Il-8(6-77 (Ser-Il-872 Gcp/Il-8 Protein I Il8/Nap1 Form Iii Lymphocyte-Derived Neutrophil-Activating Factor Lynap Mdncf-C Neutrophil-Activating Factor Naf - Il-8(7-77 Gcp/Il-8 Protein V Il8/Nap1 Form Iv - Il-8(8-77 Gcp/Il-8 Protein Vi Il8/Nap1 Form V - Il-8(9-77 Gcp/Il-8 Protein Iii Il8/Nap1 Form Vi |
| Database Links | Reactome: R-HSA-2559582 Reactome: R-HSA-375276 Reactome: R-HSA-380108 Reactome: R-HSA-380994 Reactome: R-HSA-418594 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Interleukin-8 antibody Anti-Il-8 antibody Anti-C-X-C Motif Chemokine 8 antibody Anti-Chemokine antibody Anti-C-X-C Motif Ligand 8 antibody Anti-Emoctakin antibody Anti-Granulocyte Chemotactic Protein 1 antibody Anti-Gcp-1 antibody Anti-Monocyte-Derived Neutrophil Chemotactic Factor antibody Anti-Mdncf antibody Anti-Monocyte-Derived Neutrophil-Activating Peptide antibody Anti-Monap antibody Anti-Neutrophil-Activating Protein 1 antibody Anti-Nap-1 antibody Anti-Protein 3-10c antibody Anti-T-Cell Chemotactic Factor Cleaved Into - Mdncf-A antibody Anti-Gcp/Il-8 Protein Iv antibody Anti-Il8/Nap1 Form I - Interleukin-8 antibody Anti-(Ala-Il-877 antibody Anti-Gcp/Il-8 Protein Ii antibody Anti-Il-8(1-77 antibody Anti-Il8/Nap1 Form Ii antibody Anti-Mdncf-B - Il-8(5-77 - Il-8(6-77 antibody Anti-(Ser-Il-872 antibody Anti-Gcp/Il-8 Protein I antibody Anti-Il8/Nap1 Form Iii antibody Anti-Lymphocyte-Derived Neutrophil-Activating Factor antibody Anti-Lynap antibody Anti-Mdncf-C antibody Anti-Neutrophil-Activating Factor antibody Anti-Naf - Il-8(7-77 antibody Anti-Gcp/Il-8 Protein V antibody Anti-Il8/Nap1 Form Iv - Il-8(8-77 antibody Anti-Gcp/Il-8 Protein Vi antibody Anti-Il8/Nap1 Form V - Il-8(9-77 antibody Anti-Gcp/Il-8 Protein Iii antibody Anti-Il8/Nap1 Form Vi antibody Anti-CXCL8 antibody Anti-IL8 antibody |
Information sourced from Uniprot.org