Anti-CUL7 antibody (1570-1650 aa) (STJ191692)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 1570-1650
General Information
| Short Description | Rabbit polyclonal anti-Cullin-7 (1570-1650 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CUL7 |
| Gene ID | 9820 |
| Uniprot ID | CUL7_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 1570-1650 |
| Immunogen Region | 1570-1650 aa |
| Specificity | CUL7 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | According to a report, may not be neddylated despite the conserved consensus site for neddylation at Lys-1576. Structural study of the Cul7-RING(FBXW8) reveals that both CUL7 and RBX1 are in orientations that are incompatible with neddylation. |
| Function | Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. The Cul7-RING(FBXW8) complex alone lacks ubiquitination activity and does not promote polyubiquitination and proteasomal degradation of p53/TP53. However it mediates recruitment of p53/TP53 for ubiquitination by neddylated CUL1-RBX1. Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5. The Cul7-RING(FBXW8) complex also mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1. Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain. Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development. While the Cul7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING(FBXW8) complex may have additional functions. Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation. |
| Protein Name | Cullin-7Cul-7 |
| Database Links | Reactome: R-HSA-381038Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomePerinuclear RegionGolgi ApparatusColocalizes With Fbxw8 At The Golgi Apparatus In NeuronsLocalization To Golgi Is Mediated By Obsl1During MitosisLocalizes To The Mitotic ApparatusCcdc8 Is Required For Centrosomal Location |
| Alternative Antibody Names | Anti-Cullin-7 antibodyAnti-Cul-7 antibodyAnti-CUL7 antibodyAnti-KIAA0076 antibody |
Information sourced from Uniprot.org