• Western blot analysis of extracts of various cell lines, using CTSH antibody (STJ27321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CTSH antibody (23-140) (STJ27321)

SKU:
STJ27321

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTSH
Gene ID: 1512
Uniprot ID: CATH_HUMAN
Immunogen Region: 23-140
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 23-140 of human CTSH (NP_004381.2).
Immunogen Sequence: AELCVNSLEKFHFKSWMSKH RKTYSTEEYHHRLQTFASNW RKINAHNNGNHTFKMALNQF SDMSFAEIKHKYLWSEPQNC SATKSNYLRGTGPYPPSVDW RKKGNFVSPVKNQGACGS
Function Important for the overall degradation of proteins in lysosomes.
Protein Name Pro-Cathepsin H Cleaved Into - Cathepsin H Mini Chain - Cathepsin H - Cathepsin H Heavy Chain - Cathepsin H Light Chain
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-5683826
Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Pro-Cathepsin H Cleaved Into - Cathepsin H Mini Chain - Cathepsin H - Cathepsin H Heavy Chain - Cathepsin H Light Chain antibody
Anti-CTSH antibody
Anti-CPSB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance