• Immunohistochemistry analysis of CTSF in paraffin-embedded human breast cancer using CTSF Rabbit polyclonal antibody (STJ112496) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CTSF Rabbit polyclonal antibody (STJ112496) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CTSF antibody (270-484) (STJ112496)

SKU:
STJ112496

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CTSF (270-484) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTSF
Gene ID: 8722
Uniprot ID: CATF_HUMAN
Immunogen Region: 270-484
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-484 of human CTSF (NP_003784.2).
Immunogen Sequence: LAPPEWDWRSKGAVTKVKDQ GMCGSCWAFSVTGNVEGQWF LNQGTLLSLSEQELLDCDKM DKACMGGLPSNAYSAIKNLG GLETEDDYSYQGHMQSCNFS AEKAKVYINDSVELSQNEQK LAAWLAKRGPISVAINAFGM QFYRHGISRPLRPLCSPWLI DHAVLLVGYGNRSDVPFWAI KNSWGTDWGEKGYYYLHRGS GACGVNTMASSAVVD
Tissue Specificity High expression levels in heart, skeletal muscle, brain, testis and ovary.moderate levels in prostate, placenta, liver and colon.and no detectable expression in peripheral leukocytes and thymus.
Function Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Protein Name Cathepsin F
Catsf
Database Links Reactome: R-HSA-2132295
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Cathepsin F antibody
Anti-Catsf antibody
Anti-CTSF antibody

Information sourced from Uniprot.org

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