Anti-CTLA4 antibody (49-223) (STJ115900)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CTLA4 (49-223) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CTLA4 |
| Gene ID | 1493 |
| Uniprot ID | CTLA4_HUMAN |
| Immunogen Region | 49-223 |
| Immunogen Sequence | SSRGIASFVCEYASPGKATE VRVTVLRQADSQVTEVCAAT YMMGNELTFLDDSICTGTSS GNQVNLTIQGLRAMDTGLYI CKVELMYPPPYYLGIGNGTQ IYVIDPEPCPDSDFLLWILA AVSSGLFFYSFLLTAVSLSK MLKKRSPLTTGVYVKMPPTE PECEKQFQPYFIPIN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 49-223 of human CD152/CTLA-4 (NP_005205.2). |
Additional Info
| Tissue Specificity | Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation. |
| Post Translational Modifications | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
| Function | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
| Protein Name | Cytotoxic T-Lymphocyte Protein 4Cytotoxic T-Lymphocyte-Associated Antigen 4Ctla-4Cd Antigen Cd152 |
| Database Links | Reactome: R-HSA-389356Reactome: R-HSA-389513Reactome: R-HSA-8877330 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization |
| Alternative Antibody Names | Anti-Cytotoxic T-Lymphocyte Protein 4 antibodyAnti-Cytotoxic T-Lymphocyte-Associated Antigen 4 antibodyAnti-Ctla-4 antibodyAnti-Cd Antigen Cd152 antibodyAnti-CTLA4 antibodyAnti-CD152 antibody |
Information sourced from Uniprot.org