• Western blot analysis of recombinant Human CTLA-4 Protein, using CD152/CTLA-4 Rabbit monoclonal antibody (STJ11104958) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD152/CTLA-4 using CD152/CTLA-4 Rabbit monoclonal antibody (STJ11104958) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CTLA4 antibody (37-162) [S4958RM] (STJ11104958)

SKU:
STJ11104958

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytotoxic T-Lymphocyte Protein 4 (37-162) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4958RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTLA4
Gene ID: 1493
Uniprot ID: CTLA4_HUMAN
Immunogen Region: 37-162
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-162 of human CD152/CTLA-4 (NP_005205.2).
Immunogen Sequence: AMHVAQPAVVLASSRGIASF VCEYASPGKATEVRVTVLRQ ADSQVTEVCAATYMMGNELT FLDDSICTGTSSGNQVNLTI QGLRAMDTGLYICKVELMYP PPYYLGIGNGTQIYVIDPEP CPDSDF
Tissue Specificity Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.
Post Translational Modifications N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Protein Name Cytotoxic T-Lymphocyte Protein 4
Cytotoxic T-Lymphocyte-Associated Antigen 4
Ctla-4
Cd Antigen Cd152
Database Links Reactome: R-HSA-389513
Reactome: R-HSA-8877330
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Exists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization
Alternative Antibody Names Anti-Cytotoxic T-Lymphocyte Protein 4 antibody
Anti-Cytotoxic T-Lymphocyte-Associated Antigen 4 antibody
Anti-Ctla-4 antibody
Anti-Cd Antigen Cd152 antibody
Anti-CTLA4 antibody
Anti-CD152 antibody

Information sourced from Uniprot.org

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