Anti-CTLA4 antibody (36-161aa) [AT4C8] (STJA0041945)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenRecombinant human CTLA4 (36-161aa) purified from E. coli
General Information
| Short Description | Mouse monoclonal anti-CTLA4 (36-161aa) for use in ELISA and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | AT4C8 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | By protein-G affinity chromatography |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CTLA4 |
| Gene ID | 1493 |
| Uniprot ID | CTLA4_HUMAN |
| Accession Number | NP_005205 |
| Immunogen | Recombinant human CTLA4 (36-161aa) purified from E. coli |
| Immunogen Region | 36-161aa |
Additional Info
| Tissue Specificity | Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation. |
| Post Translational Modifications | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
| Function | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
| Protein Name | Cytotoxic T-Lymphocyte Protein 4Cytotoxic T-Lymphocyte-Associated Antigen 4Ctla-4Cd Antigen Cd152 |
| Database Links | Reactome: R-HSA-389356Reactome: R-HSA-389513Reactome: R-HSA-8877330 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization |
| Alternative Antibody Names | Anti-Cytotoxic T-Lymphocyte Protein 4 antibodyAnti-Cytotoxic T-Lymphocyte-Associated Antigen 4 antibodyAnti-Ctla-4 antibodyAnti-Cd Antigen Cd152 antibodyAnti-CTLA4 antibodyAnti-CD152 antibody |
Information sourced from Uniprot.org