Anti-CTCFL antibody (STJA0009492)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human BORIS
General Information
| Short Description | Rabbit polyclonal anti-Transcriptional repressor CTCFL for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CTCFL |
| Gene ID | 140690 |
| Uniprot ID | CTCFL_HUMAN |
| Immunogen | Synthesized peptide derived from human BORIS |
| Specificity | This antibody detects endogenous levels of BORIS at Human, Mouse |
Additional Info
| Function | Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters. |
| Protein Name | Transcriptional Repressor CtcflBrother Of The Regulator Of Imprinted SitesCcctc-Binding FactorCtcf ParalogCtcf-Like ProteinCancer/Testis Antigen 27Ct27Zinc Finger Protein Ctcf-T |
| Database Links | |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Transcriptional Repressor Ctcfl antibodyAnti-Brother Of The Regulator Of Imprinted Sites antibodyAnti-Ccctc-Binding Factor antibodyAnti-Ctcf Paralog antibodyAnti-Ctcf-Like Protein antibodyAnti-Cancer/Testis Antigen 27 antibodyAnti-Ct27 antibodyAnti-Zinc Finger Protein Ctcf-T antibodyAnti-CTCFL antibodyAnti-BORIS antibody |
Information sourced from Uniprot.org