• CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 105 K562 cells with 2 ug CTCF rabbit monoclonal antibody, along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of CTCF in representative gene loci (MYC) , as shown in figure.
  • Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells and CTCF rabbit monoclonal antibody (STJ11101715). The ChIP sequencing results indicate the enrichment pattern of CTCF in selected genomic region and representative gene loci (MYC) , as shown in figure.
  • Western blot analysis of extracts of various cell lines, using CTCF antibody (STJ11101715) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Confocal imaging of U-2 OS cells using CTCF rabbit monoclonal antibody (STJ11101715, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g CTCF antibody (STJ11101715). Western blot was performed from the immunoprecipitate using CTCF antibody (STJ11101715) at a dilution of 1:1000.
  • Chromatin immunoprecipitation analysis of extracts of 293T cells, using CTCF antibody (STJ11101715) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-CTCF antibody (628-727) [S5MR] (STJ11101715)

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STJ11101715

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CTCF (628-727) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:500-1:1000
ChIP 1:20-1:100
CUT&Tag 10⁵ cells/2 Mu gChIP-seq 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTCF
Gene ID: 10664
Uniprot ID: CTCF_HUMAN
Immunogen Region: 628-727
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 628-727 of human CTCF (P49711).
Immunogen Sequence: PAVEIEPEPEPQPVTPAPPP AKKRRGRPPGRTNQPKQNQP TAIIQVEDQNTGAIENIIVE VKKEPDAEPAEGEEEEAQPA ATDAPNGDLTPEMILSMMDR
Tissue Specificity Ubiquitous. Absent in primary spermatocytes.
Post Translational Modifications Sumoylated on Lys-74 and Lys-689.sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter.
Function Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays an important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis.
Protein Name Transcriptional Repressor Ctcf
11-Zinc Finger Protein
Ccctc-Binding Factor
Ctcfl Paralog
Database Links Reactome: R-HSA-5617472
Cellular Localisation Nucleus
Nucleoplasm
Chromosome
Centromere
May Translocate To The Nucleolus Upon Cell Differentiation
Associates With Both Centromeres And Chromosomal Arms During Metaphase
Associates With The H19 Icr In Mitotic Chromosomes
May Be Preferentially Excluded From Heterochromatin During Interphase
Alternative Antibody Names Anti-Transcriptional Repressor Ctcf antibody
Anti-11-Zinc Finger Protein antibody
Anti-Ccctc-Binding Factor antibody
Anti-Ctcfl Paralog antibody
Anti-CTCF antibody

Information sourced from Uniprot.org

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