• Western blot analysis of extracts of various cell lines, using CTBP2 antibody (STJ11102685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CTBP2 rabbit monoclonal antibody (STJ11102685) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using CTBP2 rabbit monoclonal antibody (STJ11102685) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using CTBP2 rabbit monoclonal antibody (STJ11102685) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using CTBP2 rabbit monoclonal antibody (STJ11102685) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using CTBP2 rabbit monoclonal antibody (STJ11102685) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using CTBP2 rabbit monoclonal antibody (STJ11102685) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g CTBP2 antibody (STJ11102685). Western blot was performed from the immunoprecipitate using CTBP2 (STJ11102685) at a dilution of 1:1000.

Anti-CTBP2 antibody (346-445) [S5MR] (STJ11102685)

SKU:
STJ11102685

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CTBP2 (346-445) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTBP2
Gene ID: 1488
Uniprot ID: CTBP2_HUMAN
Immunogen Region: 346-445
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 346-445 of human CTBP2 (P56545).
Immunogen Sequence: TGRIPESLRNCVNKEFFVTS APWSVIDQQAIHPELNGATY RYPPGIVGVAPGGLPAAMEG IIPGGIPVTHNLPTVAHPSQ APSPNQPTKHGDNREHPNEQ
Tissue Specificity Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
Function Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation. Isoform 2 probably acts as a scaffold for specialized synapses.
Protein Name C-Terminal-Binding Protein 2
Ctbp2
Database Links Reactome: R-HSA-4641265
Reactome: R-HSA-5339700
Reactome: R-HSA-9662360 P56545-2
Cellular Localisation Nucleus
Synapse
Alternative Antibody Names Anti-C-Terminal-Binding Protein 2 antibody
Anti-Ctbp2 antibody
Anti-CTBP2 antibody

Information sourced from Uniprot.org

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