• Western blot analysis of extracts of various cell lines, using CtBP1 Rabbit monoclonal antibody (STJ11101368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of various cell lines, using CtBP1 Rabbit monoclonal antibody (STJ11101368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CTBP1 antibody (301-440) [S8MR] (STJ11101368)

SKU:
STJ11101368

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CtBP1 (301-440) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTBP1
Gene ID: 1487
Uniprot ID: CTBP1_HUMAN
Immunogen Region: 301-440
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 301-440 of human CtBP1 (Q13363).
Immunogen Sequence: QGPLKDAPNLICTPHAAWYS EQASIEMREEAAREIRRAIT GRIPDSLKNCVNKDHLTAAT HWASMDPAVVHPELNGAAYR YPPGVVGVAPTGIPAAVEGI VPSAMSLSHGLPPVAHPPHA PSPGQTVKPEADRDHASDQL
Tissue Specificity Expressed in germinal center B-cells.
Post Translational Modifications The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation. ADP-ribosylated.when cells are exposed to brefeldin A. Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.
Function Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.
Protein Name C-Terminal-Binding Protein 1
Ctbp1
Database Links Reactome: R-HSA-3769402
Reactome: R-HSA-3899300
Reactome: R-HSA-4641265
Reactome: R-HSA-5339700
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-C-Terminal-Binding Protein 1 antibody
Anti-Ctbp1 antibody
Anti-CTBP1 antibody
Anti-CTBP antibody

Information sourced from Uniprot.org

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