• Immunofluorescence analysis of HeLa cells using CSTA antibody (STJ27653). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of THP-1 cells, using CSTA antibody (STJ27653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CSTA antibody (1-98) (STJ27653)

SKU:
STJ27653

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CSTA (1-98) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CSTA
Gene ID: 1475
Uniprot ID: CYTA_HUMAN
Immunogen Region: 1-98
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human CSTA (NP_005204.1).
Immunogen Sequence: MIPGGLSEAKPATPEIQEIV DKVKPQLEEKTNETYGKLEA VQYKTQVVAGTNYYIKVRAG DNKYMHLKVFKSLPGQNEDL VLTGYQVDKNKDDELTGF
Tissue Specificity Expressed in the skin throughout the epidermis.
Function This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.
Protein Name Cystatin-A
Cystatin-As
Stefin-A Cleaved Into - Cystatin-A - N-Terminally Processed
Database Links Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Cystatin-A antibody
Anti-Cystatin-As antibody
Anti-Stefin-A Cleaved Into - Cystatin-A - N-Terminally Processed antibody
Anti-CSTA antibody
Anti-STF1 antibody
Anti-STFA antibody

Information sourced from Uniprot.org

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