• STJ71160 (1µg/ml) staining of A431 (A) , HeLa (B) and K562 (C) nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71160 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71160 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71160 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-CSNK1E antibody (C-Term) (STJ71160)

SKU:
STJ71160-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CSNK1E (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-5µg/ml
IF-Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Immunogen Region: C-Term
Accession Number: NP_001276841.1
Immunogen Sequence: PASQTSVPFDHLGK
Post Translational Modifications Autophosphorylated. Partially dephosphorylated by PPP5C. May be dephosphorylated by PP1.
Function Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.
Protein Name Casein Kinase I Isoform Epsilon
Cki-Epsilon
Ckie
Database Links Reactome: R-MMU-201688
Reactome: -MMU-2565942
Reactome: -MMU-380259
Reactome: -MMU-380270
Reactome: -MMU-380284
Reactome: -MMU-380320
Reactome: -MMU-5620912
Reactome: -MMU-6791226
Reactome: -MMU-8854518
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Casein Kinase I Isoform Epsilon antibody
Anti-Cki-Epsilon antibody
Anti-Ckie antibody
Anti-Csnk1e antibody

Information sourced from Uniprot.org

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