• Western blot analysis of various lysates, using CRYBB1 Rabbit polyclonal antibody (STJ11100891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CRYBB1 antibody (1-252) (STJ11100891)

SKU:
STJ11100891

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CRYBB1 (1-252) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRYBB1
Gene ID: 1414
Uniprot ID: CRBB1_HUMAN
Immunogen Region: 1-252
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human CRYBB1 (NP_001878.1).
Immunogen Sequence: MSQAAKASASATVAVNPGPD TKGKGAPPAGTSPSPGTTLA PTTVPITSAKAAELPPGNYR LVVFELENFQGRRAEFSGEC SNLADRGFDRVRSIIVSAGP WVAFEQSNFRGEMFILEKGE YPRWNTWSSSYRSDRLMSFR PIKMDAQEHKISLFEGANFK GNTIEIQGDDAPSLWVYGFS DRVGSVKVSSGTWVGYQYPG YRGYQYLLEPGDFRHWNEWG AFQPQMQSLRRLRDKQWHL
Post Translational Modifications Specific cleavages in the N-terminal arm occur during lens maturation and give rise to truncated forms, leading to impaired oligomerization and protein insolubilization.
Function Crystallins are the dominant structural components of the vertebrate eye lens.
Protein Name Beta-Crystallin B1
Beta-B1 Crystallin
Alternative Antibody Names Anti-Beta-Crystallin B1 antibody
Anti-Beta-B1 Crystallin antibody
Anti-CRYBB1 antibody

Information sourced from Uniprot.org

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