Anti-CRK antibody (C-Term) [M332] (STJA0005353)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenClone (M332) was generated from a recombinant sequence containing the C-terminal half of mouse Crk II. This sequence has high homology to human and rat Crk II.
General Information
| Short Description | Mouse monoclonal antibody anti-Crk II (C-Term) is suitable for use in Western Blot and Immunoprecipitation research applications. |
| Applications | WB/IP |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M332 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Protein A Purified |
| Dilution Range | WB 1:1000IP 1:50 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Crk |
| Gene ID | 12928 |
| Uniprot ID | CRK_MOUSE |
| Immunogen | Clone (M332) was generated from a recombinant sequence containing the C-terminal half of mouse Crk II. This sequence has high homology to human and rat Crk II. |
| Immunogen Region | C-Term |
| Specificity | This antibody detects a 40 kDa* protein corresponding to the molecular mass of Crk II on SDS-PAGE immunoblots of rat PC12 and human Jurkat cells. |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. Isoform Crk-II: Phosphorylated by KIT. Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form. |
| Function | Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Isoform CRK-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. |
| Protein Name | Adapter Molecule CrkProto-Oncogene C-CrkP38 |
| Database Links | Reactome: R-MMU-170984Reactome: -MMU-186763Reactome: -MMU-2029482Reactome: -MMU-372708Reactome: -MMU-4420097Reactome: -MMU-8849471Reactome: -MMU-8875555Reactome: -MMU-8875656Reactome: -MMU-912631 |
| Cellular Localisation | CytoplasmCell MembraneTranslocated To The Plasma Membrane Upon Cell Adhesion |
| Alternative Antibody Names | Anti-Adapter Molecule Crk antibodyAnti-Proto-Oncogene C-Crk antibodyAnti-P38 antibodyAnti-Crk antibodyAnti-Crko antibody |
Information sourced from Uniprot.org