• Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-).
  • Immunofluorescence of HEK293 cells transfected with a fusion construct of GFP with the C-terminal region of S. aureas Cas9 protein (green) that is labeled with Anti-CRISPR Cas9 (304-Cas9, 1:1000, red) and the DNA stained blue with DAPI. The yellow label is the overlapping of the Anti-Cas9 antibody and the Cas9-GFP construct. m
  • Immunofluorescence of HEK293 cells transfected with S. aureas Cas9 and labeled with Anti-CRISPR Cas9 (304-Cas9, 1:500, red).

Anti-CRISPR Cas9 antibody (C-Term) [6F7] (STJA0003839)

SKU:
STJA0003839-100

€553.13
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Host: Mouse
Applications: WB/IHC/ICC
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-CRISPR Cas9 (C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 6F7
Conjugation: Unconjugated
Isotype: IgG1
Formulation: 100 ul in PBS + 50% Glycerol and 5 mM Sodium Azide
Purification: This antibody was protein g purified culture from supernatant.
Dilution Range: WB 1:1000
IHC 1:1000-1:5000
ICC 1:500-1:5000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: C-Term
Immunogen: Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.
Background CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015).

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance