Anti-CRACR2A antibody (STJA0007299)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human CRACR2A
General Information
| Short Description | Rabbit polyclonal anti-EF-hand calcium-binding domain-containing protein 4B for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CRACR2A |
| Gene ID | 84766 |
| Uniprot ID | EFC4B_HUMAN |
| Immunogen | Synthesized peptide derived from human CRACR2A |
| Specificity | This antibody detects endogenous levels of CRACR2A at Human, Mouse |
Additional Info
| Function | Isoform 1: Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex. Isoform 2: Rab GTPase that mediates the trafficking of Weibel-Palade bodies (WPBs) to microtubule organizing center (MTOC) in endothelial cells in response to acute inflammatory stimuli. During histamine (but not thrombin) stimulation of endothelial cells, the dynein-bound form induces retrograde transport of a subset of WPBs along microtubules to the MTOC in a Ca(2+)-independent manner and its GTPase activity is essential for this function. Ca(2+)-regulated dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules and regulates endosomal trafficking of CD47. Acts as an intracellular signaling module bridging two important T-cell receptor (TCR) signaling pathways, Ca(2+)-NFAT and JNK, to affect T-cell activation. In resting T-cells, is predominantly localized near TGN network in a GTP-bound form, upon TCR stimulation, localizes at the immunological synapse via interaction with VAV1 to activate downstream Ca(2+)-NFAT and JNK signaling pathways. Plays a role in T-helper 1 (Th1) cell differentiation and T-helper 17 (Th17) cell effector function. Plays a role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. |
| Protein Name | Ef-Hand Calcium-Binding Domain-Containing Protein 4bCalcium Release-Activated Calcium Channel Regulator 2aCrac Channel Regulator 2aCalcium Release-Activated Channel Regulator 2aRas-Related Protein Rab-46 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: CytoplasmIsoform 2: CytoplasmCytoplasmCytoskeletonMicrotubule Organizing CenterCell MembraneGolgi Apparatus MembraneGolgi ApparatusTrans-Golgi Network MembraneVesicleT-Cell Activation-Induced Elevation Of Intracellular Of Ca(2+) Stimulates Its Transport Toward The Microtubule Organizing Center (Mtoc)Histamine Stimulation Induces A Trafficking To The Mtoc In A Gtp-Binding-Dependent But Ca(2+)-Binding-Independent MannerLocalizes To Golgi Membrane In Resting T-Cells And Upon Its Interaction With Vav1Is Translocated From The Golgi Membrane To The Immunological Synapse Via Subsynaptic VesiclesIts Localization In The Golgi Membrane Requires Isoprenylation And Gtp-Binding |
| Alternative Antibody Names | Anti-Ef-Hand Calcium-Binding Domain-Containing Protein 4b antibodyAnti-Calcium Release-Activated Calcium Channel Regulator 2a antibodyAnti-Crac Channel Regulator 2a antibodyAnti-Calcium Release-Activated Channel Regulator 2a antibodyAnti-Ras-Related Protein Rab-46 antibodyAnti-CRACR2A antibodyAnti-EFCAB4B antibodyAnti-RAB46 antibody |
Information sourced from Uniprot.org