• Western blot analysis of extracts of rat spleen, using CPSF73 antibody (STJ11103478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using CPSF73 antibody (STJ11103478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CPSF73 antibody (585-684) [S8MR] (STJ11103478)

SKU:
STJ11103478

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CPSF73 (585-684) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CPSF3
Gene ID: 51692
Uniprot ID: CPSF3_HUMAN
Immunogen Region: 585-684
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 585-684 of human CPSF3 (Q9UKF6).
Immunogen Sequence: LEVQSNPKIRKGAVQKVSKK LEMHVYSKRLEIMLQDIFGE DCVSVKDDSILSVTVDGKTA NLNLETRTVECEEGSEDDES LREMVELAAQRLYEALTPVH
Post Translational Modifications Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.
Function Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for entering/progressing through S-phase of the cell cycle. Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1. Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a. Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively.
Protein Name Cleavage And Polyadenylation Specificity Factor Subunit 3
Cleavage And Polyadenylation Specificity Factor 73 Kda Subunit
Cpsf 73 Kda Subunit
Mrna 3'-End-Processing Endonuclease Cpsf-73
Database Links Reactome: R-HSA-159231
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Reactome: R-HSA-77595
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cleavage And Polyadenylation Specificity Factor Subunit 3 antibody
Anti-Cleavage And Polyadenylation Specificity Factor 73 Kda Subunit antibody
Anti-Cpsf 73 Kda Subunit antibody
Anti-Mrna 3'-End-Processing Endonuclease Cpsf-73 antibody
Anti-CPSF3 antibody
Anti-CPSF73 antibody

Information sourced from Uniprot.org

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