• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g CPSF3L antibody (STJ28649). Western blot was performed from the immunoprecipitate using CPSF3L antibody (STJ28649) at a dilution of 1:500.
  • Immunofluorescence analysis of HeLa cells using CPSF3L Rabbit polyclonal antibody (STJ28649). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CPSF3L Rabbit polyclonal antibody (STJ28649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CPSF3L antibody (331-600) (STJ28649)

SKU:
STJ28649

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CPSF3L (331-600) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:10-1:100
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INTS11
Gene ID: 54973
Uniprot ID: INT11_HUMAN
Immunogen Region: 331-600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 331-600 of human CPSF3L (NP_060341.2).
Immunogen Sequence: GQSLQIFRKWAGNEKNMVIM PGYCVQGTVGHKILSGQRKL EMEGRQVLEVKMQVEYMSFS AHADAKGIMQLVGQAEPESV LLVHGEAKKMEFLKQKIEQE LRVNCYMPANGETVTLPTSP SIPVGISLGLLKREMAQGLL PEAKKPRLLHGTLIMKDSNF RLVSSEQALKELGLAEHQLR FTCRVHLHDTRKEQETALRV YSHLKSVLKDHCVQHLPDGS VTVESVLLQAAAPSEDPGT
Function Catalytic component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.
Protein Name Integrator Complex Subunit 11
Int11
Cleavage And Polyadenylation-Specific Factor 3-Like Protein
Cpsf3-Like Protein
Protein Related To Cpsf Subunits Of 68 Kda
Rc-68
Database Links Reactome: R-HSA-6807505
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Integrator Complex Subunit 11 antibody
Anti-Int11 antibody
Anti-Cleavage And Polyadenylation-Specific Factor 3-Like Protein antibody
Anti-Cpsf3-Like Protein antibody
Anti-Protein Related To Cpsf Subunits Of 68 Kda antibody
Anti-Rc-68 antibody
Anti-INTS11 antibody
Anti-CPSF3L antibody
Anti-RC68 antibody

Information sourced from Uniprot.org

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