• Western blot analysis of extracts of various cell lines, using CPE antibody (STJ27411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CPE antibody (257-476) (STJ27411)

SKU:
STJ27411

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CPE (257-476) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CPE
Gene ID: 1363
Uniprot ID: CBPE_HUMAN
Immunogen Region: 257-476
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 257-476 of human CPE (NP_001864.1).
Immunogen Sequence: PYDETRSGSAHEYSSSPDDA IFQSLARAYSSFNPAMSDPN RPPCRKNDDDSSFVDGTTNG GAWYSVPGGMQDFNYLSSNC FEITVELSCEKFPPEETLKT YWEDNKNSLISYLEQIHRGV KGFVRDLQGNPIANATISVE GIDHDVTSAKDGDYWRLLIP GNYKLTASAPGYLAITKKVA VPYSPAAGVDFELESFSERK EEEKEELMEWWKMMSETLNF
Function Sorting receptor that directs prohormones to the regulated secretory pathway. Acts also as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage.
Protein Name Carboxypeptidase E
Cpe
Carboxypeptidase H
Cph
Enkephalin Convertase
Prohormone-Processing Carboxypeptidase
Database Links Reactome: R-HSA-264876
Cellular Localisation Isoform 1: Cytoplasmic Vesicle
Secretory Vesicle
Cytoplasmic Vesicle
Secretory Vesicle Membrane
Peripheral Membrane Protein
Secreted
Associated With The Secretory Granule Membrane Through Direct Binding To Lipid Rafts In Intragranular Conditions
Alternative Antibody Names Anti-Carboxypeptidase E antibody
Anti-Cpe antibody
Anti-Carboxypeptidase H antibody
Anti-Cph antibody
Anti-Enkephalin Convertase antibody
Anti-Prohormone-Processing Carboxypeptidase antibody
Anti-CPE antibody

Information sourced from Uniprot.org

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