Anti-COX5A antibody [PT1270R] (STJA0040086)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgGk
STJA0040086
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General Information

Short DescriptionRabbit monoclonal anti-Cytochrome C Oxidase Subunit 5a-Mitochondrial for use in WB, IHC, IF, IP and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/IP/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDPT1270R
IsotypeIgGk
ConjugationUnconjugated
PurificationProtein A
Dilution RangeIHC 1:1000-1:400
WB 1:2000-1:10000
IF 1:200-1:1000
ELISA 1:5000-1:20000
IP 1:50-1:200
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCOX5A
Gene ID9377
Uniprot IDCOX5A_HUMAN
SpecificityEndogenous

Additional Info

Function Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Protein Name Cytochrome C Oxidase Subunit 5a - Mitochondrial
Cytochrome C Oxidase Polypeptide Va
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9707564
Reactome: R-HSA-9837999
Reactome: R-HSA-9864848
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Cytochrome C Oxidase Subunit 5a - Mitochondrial antibody
Anti-Cytochrome C Oxidase Polypeptide Va antibody
Anti-COX5A antibody

Information sourced from Uniprot.org

Citations

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